Research Article
Evaluation of Subchronic Toxicity and Genotoxicity of Ethanolic Extract of Aster glehni Leaves and Stems
Table 7
In vitro chromosome aberration test in Chinese hamster lung cells with EAG.
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Significant difference at levels compared with the negative control by Fisher’s exact test. aTreatment time – recovery time, hours, bGap excludes, 150 metaphases were examined per culture. cAfter harvesting mitotic cells, each culture was trypsinized and suspended with 0.5 mL of 0.1% trypsin and 5 mL of culture medium. The cell suspensions of 0.4 mL/culture were diluted 50 times with 19.6 mL of Isoton® sol. The cells in 0.5 mL of Isoton® sol. were counted twice/culture using Coulter Counter model Z2. The actual number of cells per flask = mean cell count × 550. dRelative increase in cell count = ((cell count of treated flask – initial cell count)/(cell count of the negative control flask – initial cell count)) × 100, PP, polyploid; ER, endoreduplication; B[a]P, benzo[a]pyrene (positive control); and 4NQO, 4-nitroquinoline-1-oxide (positive control). |