Research Article
Quercetin Improves Mitochondrial Function and Inflammation in H2O2-Induced Oxidative Stress Damage in the Gastric Mucosal Epithelial Cell by Regulating the PI3K/AKT Signaling Pathway
Figure 1
Que alleviates H2O2-induced oxidative stress, apoptosis, and inflammatory in GES-1 cells. (a) CCK-8 was performed to measure the viability of GES-1 cells. Flow cytometry was performed to evaluate the proportion of (b) cells with increase in ROS and (c) apoptotic cells. (d) Western blot was performed to detect the apoptosis-related proteins Bax, Bcl-2, and cleaved caspase 3 expression in GES-1 cells. qRT-PCR was performed to detect mRNA expression of (e) IL-10 and (f) TNF-α in GES-1 cells. Data presets as mean ± SD, n− = 3, ∗P < 0.05 vs. control group, #P < 0.05 vs. model group.
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