Research Article
Breviscapine Participates in the Progression of Prostate Cancer by Inhibiting ZFP91 Expression through Upregulation of MicroRNA-129-5p
Figure 3
ZFP91 is a potential target gene for miR-129-5p. (a) Bioinformatics analysis revealed that miR-129-5p has a potential binding site with ZFP91. (b, c) The results of the dual-luciferase reporter gene assay confirmed the binding relationship between the two. (d) After transfection of miR-129-5p inhibitor in LNCap and PC3 cells, miR-129-5p expression was significantly reduced. (e, f) The mRNA level of ZFP91 was significantly decreased after upregulating the expression of miR-129-5p in LNCap and PC3 cells; the mRNA level of ZFP91 was significantly increased after downregulating the expression of miR-129-5p (, , and ).
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