Hypoxia Upregulates NOTCH3 Signaling Pathway to Promote Endothelial-Mesenchymal Transition in Pulmonary Artery Endothelial Cells

Figure 1

EnMT in endothelial cells induced by hypoxia. (a) Observation of endothelial cell morphology. (b) Detection of the expression of VE-cadherin and Vimentin by western blot experiment. (c) Histogram of protein expression. (d) Detection of the cell viability of endothelial cells by MTT analysis. (e) Transwell assay to evaluate cell migration ability. (f) Scratch test to evaluate cell migration ability. (g) The fluorescent microscope of immunofluorescence staining shows the intensity of α-SMA antibody staining. DAPI was used for nuclear staining. (h) The fluorescent microscope of immunofluorescence staining shows vWF expression detection. DAPI was used for nuclear staining. , Student's t-test. All data are expressed as the mean ± SD of three replicate experiments.