Research Article

Preliminary Study on the In Vitro Antitumor Effects of Nidus Vespae on Gastric Cancer

Figure 2

Effects of NVD on the apoptosis rate and cell cycle distribution in SGC-7901 cells. (a) Detection of apoptosis in SGC-7901 cells by flow cytometry. The cells were treated with various concentrations of NVD at 20, 30, and 40 mg/mL for 48 h and then stained with annexin V-FITC/PI and analyzed by flow cytometry. (b) The apoptosis rate of SGC-7901 cells in each group. The total apoptosis rate = percentage of early apoptosis + percentage of late apoptosis. (c) DNA content in SGC-7901 cells treated with various concentrations of NVD. After NVD treatment for 48 h, the cells were stained with PI and analyzed by flow cytometry. (d) Proportions of cells in each cell cycle stage based on the DNA content. The data are mean ± SD, n = 3. , , and , compared to the control.
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