Preliminary Study on the In Vitro Antitumor Effects of Nidus Vespae on Gastric Cancer

Figure 3

Low concentrations of NVD stimulate THP-1 proliferation and differentiation. (a) Proliferation rate (%) of THP-1 after NVD stimulation (0.312, 0.625, 1.250, 2.5, 5, 10, and 20 mg/mL) for 48 h. The solid and dashed lines represent culture of THP-1 in medium supplemented with 10% FBS and serum-free medium, respectively. The data are mean ± SD of three separate experiments using different batches of cells. All experiments were performed in triplicate. Data were analyzed by two-way ANOVA with the Bonferroni post hoc test (, , and , compared to PBS control). (b) THP-1 cells were cultured with 5 mg/mL NVD for 48 h and cell morphology was observed under a microscope. (A) THP-1 cells in medium supplemented with 10% FBS as the control; (B) THP-1 cultured with 5 μg/mL NVD in medium supplemented with 10% FBS; (C) THP-1 in serum-free medium (control); (D) THP-1 cultured with 5 mg/mL NVD in serum-free medium.

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