Preliminary Study on the In Vitro Antitumor Effects of Nidus Vespae on Gastric Cancer

Figure 4

Effects of NVD on THP-1 function. (a) Secretion of proinflammatory cytokines induced by THP-1 after culturing with 5 mg/mL NVD in serum-free medium, as revealed by ELISA. (b) The expression of THP-1 DC surface markers was assessed by flow cytometry. The light gray peaks represent isotype control, the medium gray peaks represent medium only, the dark gray peaks represent 5 mg/mL NVD, and the dark peaks represent 10 mg/mL NVD. (c) Flow cytometry analysis of the endocytotic activity of THP-1 DCs. Uptake of 40 kDa dextran was measured after 10 min. Light gray peak: autofluorescence of THP-1 cells not incubated with 40 kDa dextran. Medium gray peak: control; dark gray peak: 5 mg/mL NVD; black peak: 10 mg/mL NVD. A histogram representing three independent experiments with similar results is shown. .

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