Polyphyllin I Inhibits Propionibacterium acnes-Induced IL-8 Secretion in HaCaT Cells by Downregulating the CD36/NOX1/ROS/NLRP3/IL-1β Pathway

Figure 1

P. acnes inducing IL-8 release and the activation of NLRP3 inflammasome in HaCaT cells. (a) The CD36, NOX1, NLRP1, NLRP3, NLRC4, AIM2, ASC, active caspase-1, and cleaved IL-1β protein levels in HaCaT cells following 10⁸ CFU/ml P. acnes treatment detection done by Western blot. GAPDH was used as the loading control. CD36, NOX1 (b), NLRP1, NLRP3 (c), NLRC4, AIM2 (d), and ASC (e) relative protein levels compared to the GAPDH level, active caspase-1/pro-caspase-1, and cleaved IL-1β/pro-IL-1β are calculated by ImageJ. (h) ELISA was performed for the analysis of IL-8 protein levels in the culture supernatant of HaCaT cells. n = 5/each group. and vs. 0 h group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)