Research Article
Polyphyllin I Inhibits Propionibacterium acnes-Induced IL-8 Secretion in HaCaT Cells by Downregulating the CD36/NOX1/ROS/NLRP3/IL-1β Pathway
Figure 2
P. acnes inducing the CD36/NOX1/ROS/NLRP3/IL-1β/IL-8 pathway in HaCaT cells. (a) The CD36, NOX1, NLRP3, ASC, active caspase-1, and cleaved IL-1β protein levels in HaCaT cells following 108 CFU/ml P. acnes treatment with CD36 siRNA, NOX1 siRNA, DPI, NAC, NC siRNA, or 01% DMSO exposure detected by Western blot. GAPDH is used as the loading control. CD36, NOX1 (b), NLRP3, and ASC (c) relative protein levels compared to GAPDH expression, active caspase-1/procaspase-1 (d), and cleaved IL-1β/pro-IL-1β (e) calculated by ImageJ. (f) The IL-8 protein levels in the culture supernatant of HaCaT cells determined by ELISA. (g) Cytofluorometric profiles representing the distribution of HaCaT cells after staining with DCFH-DA. (h) ROS positive ratio calculated by cytometry flow. n = 5/each group. vs. control group. vs. P. acnes treatment group.
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