Effects of Nigella sativa on Performance, Blood Profiles, and Antibody Titer against Newcastle Disease in Broilers

Table 3

Effects of N. sativa on hemogram (mean ± SEM) of broiler chickens.

Age (day)	Group	RBC (×106)	PCV (%)	Hb (g/dl)
Day1	T0−T7	4.34 ± 0.029NS	24.2 ± 0.00NS	9.68 ± 0.047NS
Day 7	T0	5.00 ± 0.054a	27.2 ± 0.00a	10.98 ± 0.015a
	T1	5.44 ± 0.024a	31.0 ± 0.00a	11.40 ± 0.044a
	T2	5.5 ± 0.024a	31.8 ± 0.00a	11.64 ± 0.024a
	T3	5.54 ± 0.024a	31.6 ± 0.00a	11.60 ± 0.044a
	T4	5.52 ± 0.040a	30.6 ± 0.00a	11.20 ± 0.063a
	T5	5.50 ± 0.019a	29.8 ± 0.00a	11.08 ± 0.051a
	T6	5.46 ± 0.015a	29.3 ± 0.00a	10.92 ± 0.020a
	T7	5.42 ± 0.048a	29.0 ± 0.00a	10.7 8 ± 0.020a
Day 14	T0	4.32 ± 0.048a	26.6 ± 0.00a	8.28 ± 0.074a
	T1	4.34 ± 0.060a	26.8 ± 0.00ab	8.36 ± 0.021ab
	T2	4.44 ± 0.024a	27.4 ± 0.00a	8.56 ± 0.067ab
	T3	4.48 ± 0.020abc	27.8 ± 0.00b	8.70 ± 0.080b
	T4	4.50 ± 0.067b	27.8 ± 0.00b	8.72 ± 0.019b
	T5	4.54 ± 0.064bcd	28.0 ± 0.00bc	8.76 ± 0.015bc
	T6	4.62 ± 0.073cb	28.6 ± 0.00cbd	8.96 ± 0.018bc
	T7	4.76c ± 0.024d	29.6 ± 0.01d	9.28 ± 0.073d
Day 21	T0	4.62 ± 0.048a	28.6 ± 0.00a	8.98 ± 0.073a
	T1	4.64 ± 0.060a	28.8 ± 0.00a	9.04 ± 0.019ab
	T2	4.74 ± 0.024ab	29.4 ± 0.00ba	9.22 ± 0.073a
	T3	4.78 ± 0.020ac	29.8 ± 0.00b	9.34 ± 0.060bcde
	T4	4.80 ± 0.067bcd	29.8 ± 0.00bc	9.34 ± 0.018c
	T5	4.84 ± 0.087bc	30.0 ± 0.00bcd	9.4 ± 0.016bcd
	T6	4.94 ± 0.048cb	30.6 ± 0.00cbd	9.58 ± 0.014dce
	T7	5.12 ± 0.031d	31.6 ± 0.00d	9.88 ± 0.073e
Day 28	T0	4.78 ± 0.024a	29.6 ± 0.00a	9.28 ± 0.073a
	T1	4.80 ± 0.054ab	29.8 ± 0.00ab	9.30 ± 0.008ab
	T2	4.88 ± 0.048ab	30.4 ± 0.00abc	9.52 ± 0.073abc
	T3	4.96 ± 0.040ac	30.8 ± 0.00bc	9.64 ± 0.060bc
	T4	4.98 ± 0.059bc	30.8 ± 0.00cd	9.64 ± 0.009cd
	T5	5.00 ± 0.001bc	31.0 ± 0.00bcd	9.7 ± 0.007bcd
	T6	5.10 ± 0.057cd	31.8 ± 0.00dce	9.88 ± 0.008dce
	T7	5.26 ± 0.024db	32.6 ± 0.00edc	10.18 ± 0.073edc
Day 35	T0	5.12 ± 0.048a	31.6 ± 0.00a	9.88 ± 0.073a
	T1	5.14 ± 0.060a	31.8 ± 0.00ab	9.94 ± 0.000ab
	T2	5.24 ± 0.024 ab	32.4 ± 0.00abc	10.12 ± 0.073ab
	T3	5.28 ± 0.020 abc	32.8 ± 0.00bcd	10.24 ± 0.060bcd
	T4	5.30 ± 0.067bc	33.0 ± 0.00cd	10.24 ± 0.008c
	T5	5.34 ± 0.074bcd	33.0 ± 0.00cbd	10.30 ± 0.007c
	T6	5.42 ± 0.073cd	33.6 ± 0.00dc	10.48 ± 0.009dce
	T7	5.56 ± 0.024dc	34.6 ± 0.00ed	10.78 ± 0.073ed
Day 42	T0	5.56 ± 0.024a	34.6 ± 0.01a	10.75 ± 0.07a
	T1	5.60 ± 0.054abc	34.8 ± 0.01ab	10.86 ± 0.01abc
	T2	5.68 ± 0.048ab	35.4 ± 0.01ab	11.06 ± 0.09abc
	T3	5.76 ± 0.040abcd	35.8 ± 0.01bcd	11.20 ± 0.08bc
	T4	5.78 ± 0.080bc	35.8 ± 0.01bc	11.22 ± 0.01bc
	T5	5.80 ± 0.010bc	36.0 ± 0.01bc	11.26 ± 0.01bcd
	T6	5.90 ± 0.080cd	36.6 ± 0.01cd	11.46 ± 0.01bcd
	T7	6.06 ± 0.024d	37.6 ± 0.01dc	11.78 ± 0.07d

Note. RBCs: red blood cells, PCV: packed cell volume, and Hb: hemoglobin. T0 = control 1 (without vaccination and without supplementation of N. sativa in diet), T1 = control 2 (T0 + vaccination), T2 = T1 + N. sativa 0.5%, T3 = T1 + N. sativa 1%, T4 = T1 + N. sativa 2%, T5 = T1 + N. sativa 4%, T6 = T1 + N. sativa 8%, and T7 = T1 + N. sativa 16%. Different superscript letters in each column/week indicate significant () differences between groups, and NS stands for not significant ().