Effects of Nigella sativa on Performance, Blood Profiles, and Antibody Titer against Newcastle Disease in Broilers
Table 4
Effects of N. sativa on leukogram (mean ± SEM) of broiler chickens.
Age
Group
WBC (×103)
Het (%)
Lym (%)
Het/Lymp
Mon (%)
Eos (%)
Bas (%)
Day1
T0-T7
20.37 ± 0.18NS
37 ± 7NS
51.4 ± 7NS
0.94 ± 0NS
4 ± 0NS
6 ± 0NS
1.62 ± 0NS
Day 7
T0
29.1 ± 0.10a
30.0 ± 1a
55.0 ± 0.8a
0.54 ± 0.00a
4.8 ± 0.0acd
4.2 ± 0ac
6.02 ± 0a
T1
29.2 ± 0.20a
38.8 ± 1b
51.0 ± 1b
0.76 ± 0.00b
3.4 ± 0.0 b
3.22 ± 0b
3.62 ± 0b
T2
28.6 ± 0.21a
37.2 ± 1b
51.4 ± 0.5ab
0.72 ± 0.02b
4.0 ± 0.0ab
3.42 ± 0ab
4.02 ± 0b
T3
26.8 ± 0.12b
34.6 ± 1ba
52.8 ± 0.7ab
0.65 ± 0.02 b
4.2 ± 0.0abc
4.02 ± 0abc
4.42 ± 0bc
T4
25.0 ± 0.41b
31.2 ± 3ca
54.8 ± 0.0a
0.56 ± 0.03a
4.6 ± 0.0abc
4.42 ± 0c
5.02 ± 1cd
T5
24.2 ± 0.53b
27.2 ± 1cad
56.6 ± 1ca
0.48 ± 0.09a
5.0 ± 0.0c
4.62 ± 0 cd
5.62 ± 0da
T6
23.0 ± 0.10 cd
23.0 ± 2d
60.2 ± 1dc
0.38 ± 0.01c
5.4 ± 0.0 d
5.62 ± 0de
5.82 ± 1da
T7
21.0 ± 0.10 d
21.2 ± 4d
60.6 ± 2d
0.34 ± 0.00c
6.4 ± 0.0e
6.02 ± 0e
5.82 ± 0da
Day 14
T0
20.72 ± 0.48ab
47.0 ± 1a
46.0 ± 0.1a
1.02 ± 0.02a
3.7 ± 0a
2.2 ± 0a
1.62 ± 0a
T1
21.40 ± 0.50 a
45.8 ± 1a
45.8 ± 0.3a
1.00 ± 0.04a
3.8 ± 0a
2.82 ± 0a
1.82 ± 0a
T2
19.36 ± 0.72ab
43.2 ± 1ab
46.6 ± 0.5a
0.93 ± 0.02abc
4.4 ± 0ab
3.42 ± 0ab
2.42 ± 0ab
T3
18.82 ± 0.86bc
42.0 ± 1ba
46.6 ± 0.7a
0.89 ± 0.02b
4.82 ± 0bc
3.82 ± 0bc
2.82 ± 0bc
T4
18.43 ± 1.56bc
41.8 ± 3bc
46.6 ± 0.0a
0.89 ± 0.04bc
4.92 ± 0bc
3.82 ± 0bc
2.92 ± 0bc
T5
17.57 ± 0.74c
41.4 ± 1bc
46.6 ± 0.6a
0.88 ± 0.06bc
5.02 ± 0bc
4.02 ± 0bc
3.02 ± 0bc
T6
17.18 ± 1.24c
39.0 ± 2cd
47.2 ± 0.5b
0.82 ± 0.05cd
5.62 ± 0cd
4.62 ± 0 cd
3.62 ± 0 cd
T7
16.90 ± 0.58c
34.6 ± 4d
48.6 ± 0.8b
0.71 ± 0.02d
6.62 ± 0d
5.62 ± 0d
4.62 ± 0d
Day 21
T0
22.70 ± 0.04ab
33.6 ± 0a
52.6 ± 0a
0.64 ± 0.01a
4.62 ± 0a
6.62 ± 0a
2.62 ± 0a
T1
22.95 ± 0.05a
33.8 ± 0a
51.8 ± 1a
0.65 ± 0.02a
4.82 ± 0ab
6.82 ± 0ab
2.82 ± 0ab
T2
20.91 ± 0.07abc
34.4 ± 0ab
49.4 ± 0ab
0.69 ± 0.02a
5.42 ± 0abc
7.42 ± 0abc
3.42 ± 0abc
T3
20.37 ± 0.08bcd
34.6 ± 0bd
48.6 ± 0b
0.71 ± 0.01ab
5.62 ± 0bc
7.62 ± 0b
3.62 ± 0bc
T4
20.12 ± 0.10cbd
34.8 ± 0bd
47.8 ± 1bd
0.73 ± 0.03ab
5.82 ± 0cd
7.82 ± 0cd
3.82 ± 0cd
T5
19.98 ± 0.07cbd
35.0 ± 0cb
47.0 ± 2cb
0.74 ± .04ab
6.02 ± 0cd
8.02 ± 0cd
4.02 ± 0cd
T6
18.73 ± 0.12cd
35.6 ± 0dc
44.6 ± 2dce
0.80 ± 0.04bc
6.62 ± 0d
8.62 ± 0d
4.62 ± 0d
T7
18.45 ± .05d
36.6 ± 0e
40.6 ± 0e
0.90 ± 0.02c
7.6 ± 0 e
9.62 ± 0e
5.62 ± 0e
Day 28
T0
23.80 ± 0.04a
37.6 ± 0a
45.6 ± 0a
0.82 ± 0.02a
5.62 ± 0a
7.62 ± 0a
3.62 ± 0a
T1
24.50 ± 0.05ab
37.8 ± 0a
44.8 ± 1a
0.84 ± 0.03a
5.72 ± 0ab
7.92 ± 0a
3.82 ± 0a
T2
22.46 ± 0.07abc
38.4 ± 0ab
42.4 ± 0ab
0.90 ± 0.02ab
6.42 ± 0abc
8.32 ± 0ab
4.52 ± 0ab
T3
21.90 ± 0.08abc
38.5 ± 0ab
41.6 ± 0b
0.92 ± 0.02ab
6.62 ± 0bcd
8.52 ± 0ab
4.82 ± 0bc
T4
21.67 ± 0.01ac
38.8 ± 0bc
40.6 ± 2b
0.95 ± 0.05ab
6.82 ± 0cd
8.92 ± 0bc
4.92 ± 0bc
T5
21.53 ± 0.07c
39.0 ± 0bc
40.0 ± 2b
0.97 ± 0.06ab
7.02 ± 0dcd
9.02 ± 0bc
5.02 ± 0bc
T6
20.28 ± 0.12dc
39.6 ± 0c
37.6 ± 2cd
1.05 ± 0.07bc
7.52 ± 0d
9.62 ± 0 cd
5.72 ± 0c
T7
20.00 ± 0.05dc
40.6 ± 0d
33.6 ± 0d
1.20 ± 0.04c
8.52 ± 0e
10.42 ± 0d
6.92 ± 0d
Day 35
T0
25.57 ± 0.04ab
35.4 ± 0a
57.8 ± 0a
0.61 ± 0.01a
3.62 ± 0a
2.52 ± 0a
0.72 ± 0a
T1
26.25 ± 0.05a
35.8 ± 0ab
56.8 ± 1ab
0.63 ± 0.02ab
3.92 ± 0ab
2.72 ± 0ab
0.82 ± 0ab
T2
24.21 ± 0.07 abc
36.3 ± 0ab
54.5 ± 0abc
0.66 ± 0.01ab
4.32 ± 0abc
3.52 ± 0abc
1.42 ± 0abc
T3
23.67 ± 0.08abc
36.5 ± 0bc
53.8 ± 0bc
0.68 ± 0.01ab
4.62 ± 0bc
3.52 ± 0bc
1.62 ± 0abc
T4
23.42 ± 0.10bc
36.8 ± 0c
52.7 ± 1c
0.70 ± 0.03abc
4.82 ± 0bcd
3.92 ± 0cd
1.82 ± 0cd
T5
23.28 ± 0.07bc
37.0 ± 0c
52.0 ± 2c
0.71 ± 0.04abc
5.02 ± 0cd
4.02 ± 0cd
2.02 ± 0cd
T6
22.03 ± 0.12c
37.6 ± 0d
49.7 ± 2d
0.76 ± 0.04bc
5.52 ± 0d
4.62 ± 0d
2.62 ± 0de
T7
21.72 ± 0.05c
38.6 ± 0e
45.7 ± 0e
0.84 ± 0.02c
6.62 ± 0e
5.62 ± 0e
3.52 ± 0e
Day 42
T0
27.12 ± 0.04ab
33.6± ± 0a
54.7 ± 0a
0.61 ± 0.01a
3.62 ± 0a
5.52 ± 0a
2.62 ± 0a
T1
27.80 ± 0.05a
33.8± ± 0ab
53.9 ± 1ab
0.63 ± 0.02ab
3.82 ± 0ab
5.72 ± 0ab
2.82 ± 0ab
T2
25.76 ± 0.07abc
34.4± ± 0abc
51.5 ± 0abc
0.66 ± 0.01ab
4.32 ± 0abc
6.42 ± 0abc
3.42 ± 0abc
T3
25.22 ± 0.08abc
34.6 ± 0bc
50.6 ± 0bc
0.68 ± 0.01abc
4.62 ± 0bcd
6.52 ± 0bc
3.72 ± 0bcd
T4
24.83 ± 0.10bc
34.9 ± 0cd
49.7 ± 1bcd
0.70 ± 0.03abc
4.82 ± 0cd
6.82 ± 0cd
3.82 ± 0cd
T5
24.67 ± 0.07bc
35.0 ± 0cd
49.0 ± 2cd
0.71 ± 0.04bc
5.02 ± 0cd
7.02 ± 0cd
4.02 ± 0cd
T6
23.58 ± 0.12c
35.6 ± 0d
46.7 ± 2d
0.76 ± 0.04c
5.52 ± 0d
7.62 ± 0d
4.62 ± 0d
T7
23.30 ± 0.05c
36.6 ± 0e
42.5 ± 0e
0.86 ± 0.02d
6.62 ± 0e
8.72 ± 0e
5.62 ± 0e
Note. WBC: white blood cells, Het: heterophils, Lym: lymphocytes, H/L: heterophils to lymphocytes ratio, Mon: monocytes, Eos: eosinophils, and Bas: basophils. T0 = control 1 (without vaccination and without supplementation of N. sativa in diet), T1 = control 2 (T0 + vaccination), T2 = T1 + N. sativa 0.5%, T3 = T1 + N. sativa 1%, T4 = T1 + N. sativa 2%, T5 = T1 + N. sativa 4%, T6 = T1 + N. sativa 8%, and T7 = T1 + N. sativa 16%. Different superscript letters in each column/week indicate significant () differences between groups, and NS stands for not significant ().