Effects of Nigella sativa on Performance, Blood Profiles, and Antibody Titer against Newcastle Disease in Broilers
Table 6
Effects of N. sativa on biochemical parameters (Mean ± SEM) of broilers.
Age (day)
Group
Chol (mg/dL)
Trigl (mg/dL)
ALP (U/L)
ALT (U/L)
AST (U/L)
Day1
T0–T7
304.9 ± 6.7NS
179.825 ± 5.1NS
12.03 ± 0.17NS
7.9 ± 0.3NS
218.4 ± 2.6NS
Day 7
T0
154.8 ± 2.7a
137.0 ± 2.7a
10.38 ± 0.12a
9.2 ± 0.3a
241.2 ± 2.3a
T1
155.2 ± 1.9a
132.0 ± 1.8b
10.28 ± 0.22a
9.6 ± 0.4a
246.8 ± 2.6a
T2
156.2 ± 1.6a
115.8 ± 2.0c
10.03 ± 0.13a
9.8 ± 0.3a
237.6 ± 2.4b
T3
157.2 ± 1.5a
115.4 ± 2.1c
9.94 ± 0.10a
10.6 ± 0.4b
236.8 ± 2.5b
T4
155.4 ± 1.2a
112.4 ± 2.1c
9.92 ± 0.10a
11.2 ± 0.5b
233.8 ± 2.8bc
T5
155.8 ± 1.5a
107.2 ± 1.9d
9.91 ± 0.13a
11.0 ± 0.3b
228.4 ± 2.4cd
T6
154.0 ± 1.7a
105.8 ± 2.0d
9.91 ± 0.10a
9.2 ± 0.3a
227.6 ± 2.6d
T7
154.6 ± 1.3a
103.4 ± 2.4d
9.82 ± 0.10a
8.0 ± 0.4c
227.0 ± 2.2d
Day 14
T0
154.4 ± 1.3a
173.2 ± 3.4a
8.96 ± 0.14a
9.7 ± 0.4ac
234.6 ± 2.4a
T1
150.4 ± 2.4a
172.0 ± 2.7a
8.98 ± 0.20a
9.6 ± 0.3ac
234.0 ± 2.5a
T2
151.6 ± 1.7a
167.4 ± 3.1b
8.78 ± 0.10ab
10.0 ± 0.5a
230.0 ± 2.5a
T3
150.4 ± 1.5a
166.2 ± 2.9b
8.58 ± 0.10ab
10.0 ± 0.5a
224.2 ± 2.8b
T4
153.2 ± 3.1a
161.2 ± 3.5c
8.74 ± 0.10ab
11.6 ± 0.6b
194.8 ± 2.9c
T5
153.4 ± 1.9a
157.6 ± 3.7c
8.58 ± 0.16ab
9.8 ± 0.5a
181.6 ± 2.6d
T6
154.8 ± 2.5a
151.8 ± 3.1c
8.50 ± 0.10ab
9.6 ± 0.4ac
178.4 ± 2.6d
T7
151.0 ± 3.1a
145.0 ± 3.7d
8.42 ± 0.11b
9.2 ± 0.7c
149.4 ± 2.3e
Day 21
T0
167.4 ± 2.3a
163.2 ± 3.4a
6.98 ± 0.14ab
10.0 ± 0.4ab
215.6 ± 2.3a
T1
165.6 ± 3.3a
165.0 ± 2.7a
7.02 ± 0.20a
10.6 ± 0.3a
215.2 ± 2.5a
T2
163.4 ± 1.5abc
164.4 ± 4.2a
6.82 ± 0.10abc
11.0 ± 0.7a
206.6 ± 2.3ab
T3
161.4 ± 2.9abc
160.4 ± 2.9a
6.78 ± 0.10abc
11.2 ± 0.5a
200.2 ± 2.2 b
T4
160.8 ± 1.9abc
160.2 ± 4.5ab
6.60 ± 0.18abc
11.2 ± 0.6a
199.4 ± 2.3 b
T5
160.6 ± 1.9abc
158.0 ± 4.7 b
6.58 ± 0.10abc
9.8 ± 0.5 b
190.4 ± 2.9bc
T6
158.8 ± 2.5bc
151.6 ± 5.1c
6.54 ± 0.10bc
9.6 ± 0.4b
178.0 ± 2.2c
T7
157.0 ± 2.4c
144.4 ± 3.7d
6.44 ± 0.10c
8.2 ± 0.7c
148.8 ± 2.6d
Day 28
T0
156.4 ± 2.2a
159.2 ± 3.7a
6.34 ± 0.13a
10.2 ± 0.2a
191.6 ± 2.2a
T1
154.4 ± 2.3a
159.4 ± 2.7a
6.38 ± 0.20a
10.2 ± 0.2a
194.0 ± 2.3a
T2
154.4 ± 2.1a
156.4 ± 3.4ab
6.18 ± 0.10ab
10.8 ± 0.2a
184.0 ± 2.3b
T3
154.2 ± 1.5a
152.8 ± 3.1b
6.12 ± 0.10ab
11.0 ± 0.1b
181.2 ± 2.8b
T4
154.4 ± 2.9a
147.4 ± 2.9b
5.98 ± 0.10abc
11.6 ± 0.2b
175.8 ± 2.9cb
T5
148.6 ± 2.5b
138.4 ± 3.5c
5.92 ± 0.15abc
10.4 ± 0.2bac
170.6 ± 2.8c
T6
148.4 ± 2.4b
131.6 ± 3.7d
5.88 ± 0.08bc
10.0 ± 0.2bac
164.4 ± 2.8 d
T7
144.0 ± 2.4b
128.0 ± 4.1d
5.66 ± 0.09c
9.80 ± 0.1c
138.4 ± 2.2e
Day 35
T0
150.4 ± 2.3a
153.2 ± 3.7a
5.54 ± 0.13a
10.6 ± 0.1a
182.6 ± 2.4a
T1
149.4 ± 3.3a
150.0 ± 3.4ab
5.58 ± 0.20a
10.2 ± 0.2a
182.2 ± 2.2a
T2
149.6 ± 3.1a
147.8 ± 2.7b
5.38 ± 0.08ab
10.2 ± 0.2a
171.0 ± 2.8 b
T3
145.4 ± 2.9ab
142.8 ± 2.1bc
5.32 ± 0.10ab
10.4 ± 0.1a
165.6 ± 2.8bc
T4
145.8 ± 2.5ab
138.4 ± 2.9 cd
5.18 ± 0.09abc
11.8 ± 0.2b
162.8 ± 2.5cd
T5
144.8 ± 2.4ab
133.0 ± 2.5de
5.12 ± 0.15abc
10.0 ± 0.2a
157.4 ± 2.7d
T6
139.8 ± 2.2bc
130.2 ± 2.7e
5.08 ± 0.08bc
10.0 ± 0.1a
141.4 ± 2.7e
T7
136.0 ± 2.3c
126.2 ± 2.1
4.84 ± 0.08c
9.0 ± 0.1c
133.4 ± 2.0e
Day 42
T0
136.4 ± 2.1a
132.2 ± 3.4a
5.14 ± 0.13a
10.8 ± 0.1a
144.6 ± 2.5a
T1
135.4 ± 1.5a
132.0 ± 2.7a
5.16 ± 0.20a
10.6 ± 0.2a
144.2 ± 2.4a
T2
133.4 ± 1.9a
127.2 ± 2.1ab
4.98 ± 0.06ab
10.6 ± 0.2a
133.0 ± 2.4 b
T3
130.8 ± 1.9a
124.4 ± 2.9 bc
4.92 ± 0.10ab
10.8 ± 0.2a
127.6 ± 2.8bc
T4
129.6 ± 2.3a
123.4 ± 2.5bc
4.74 ± 0.09abc
12.0 ± 0.1b
124.8 ± 2.9c
T5
121.2 ± 2.4b
121.6 ± 2.7c
4.72 ± 0.15abc
10.0 ± 0.3c
119.4 ± 2.8c
T6
117.8 ± 1.5b
114.4 ± 2.1de
:
9.4 ± 0.2c
103.4 ± 2.9d
T7
108.0 ± 2.9c
110.6 ± 3.7e
4.48 ± 0.08c
8.8 ± 0.2d
95.4 ± 2.2d
Note. Chol: cholesterol, Trigl: triglyceride, ALP: alkaline phosphatase, ALT: alanine transaminase, and AST: aspartate transaminase. T0 = control 1 (without vaccination and without supplementation of N. sativa in diet), T1 = control 2 (T0 + vaccination), T2 = T1 + N. sativa 0.5%, T3 = T1 + N. sativa 1%, T4 = T1 + N. sativa 2%, T5 = T1 + N. sativa 4%, T6 = T1 + N. sativa 8%, and T7 = T1+N. sativa 16%. Different superscript letters in each column/week indicate significant () differences between groups, and NS stands for not significant ().
