Synergistic Protective Effect of Curcumin and Resveratrol against Oxidative Stress in Endothelial EAhy926 Cells

Figure 2

Individual and combined C and R restored H₂O₂ impaired cell viability in EAhy926 cells assessed by Alamar Blue and MTT assays (n = 3). Pretreatment of C, R, or CR in different ratios (1 : 9, 2 : 8, …, 9 : 1) restored the cell viability of EAhy926 cells against the stimulation of H₂O₂ at 2.5 mM assessed by Alamar Blue assay (a). Gallic acid (GA) was used as a positive control in this model. In particular, the effect of CR 8 : 2 in restoring the impaired cell viability was generally greater than that of C or R as assessed by Alamar Blue assay (c). Similar trend was found in the MTT assay that pretreatment of C, R, or CR in different ratios (1 : 9, 2 : 8, …, 9 : 1) restored the cell viability (b), and the effect of CR 8 : 2 in restoring the impaired cell viability was generally greater than that of C or R (d). , , , vs. H₂O₂ at 1 mM. ^####, ^###, ^##, ^# vs. CR 8 : 2 at the same concentration level. The error bars represent the standard deviation of measurements for three samples in three separate sample runs (n = 3). Synergistic interaction of CR in restoring cell viability analysed by CI-Fa curve (e).

(a)

(b)

(c)

(d)

(e)