Synergistic Protective Effect of Curcumin and Resveratrol against Oxidative Stress in Endothelial EAhy926 Cells

Figure 6

CR 8 : 2 synergistically increased Nrf2 and enhanced the upregulation of HO-1 protein expression (n = 3). Nrf2 protein activation of C (a), R (b), and CR 8 : 2 (c) compared to the negative control (untreated cells) measured by luciferase assay with AREc32 cell line. (d) The CI-Fa curve of CR 8 : 2 upregulating Nrf2 as analysed by CompuSyn software. (e) The comparison of Nrf2 fold increase in C, R, and CR 8 : 2 at 25 and 50 μM. (f) Representative western blot images of HO-1 protein and the quantitative expressions following the coincubation of C, R, and C-R (25 μM) in EAhy926 cells measured by western blot analysis. vs. blank; ^# vs. C or R at the same concentration level. The error bars represent the standard deviation of measurements for at least three samples in three separate assay runs (n = 3).

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