Painong San, a Traditional Chinese Compound Herbal Medicine, Restores Colon Barrier Function on DSS-Induced Colitis in Mice

Figure 4

PNS improves intestinal epithelial barrier function. (a) Colon tissue section (FITC-4 kDa submucosal infiltration, Claudin-1 IHC staining, and representative photos of ZO-1 and occludin-1 IF staining), secondary antibody was used and observed by fluorescence microscopy (red staining). Nuclei were stained with 4,6‐diamidino-2-phenylindole (DAPI; blue staining); (b) the fluorescence intensity of FITC-D in serum at 4 h after administration (n = 9); (c) statistical histogram of Claudin-1 positive expression; (d) statistical histogram of positive expression of ZO-1; (e) statistical histogram of positive expression of occludin-1; the histogram data is calculated by selecting 10 random fields of view for each mouse, n = 3 mice for each group. , , and , versus the normal group, , , and versus the DSS group.

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