Quercetin Reduces Oxidative Stress and Apoptosis by Inhibiting HMGB1 and Its Translocation, Thereby Alleviating Liver Injury in ACLF Rats

Figure 5

Effects of Que on the expression and translocation of HMGB1 in ACLF rats. (a) Immunofluorescence staining of HMGB1 expression and translocation. Arrows indicate the HMGB1 in cytoplasm. Magnification 400 (X); scale bar: 100 μm. (b) Representative immunoblots for the HMGB1 in the nucleus; HMGB1 in the cytoplasm. (c, d) HMGB1 in the cytoplasm and nucleus under different treatments under different treatment by western blot assay. (e, f) Calculated results of the ratio of HMGB1 in the cytoplasm and the total expression of HMGB1. According to the different positions of HMGB1 expressed in the cytoplasm and nucleus, GAPDH and histone H3 were selected as housekeeping proteins. Data are presented as the mean ± SD (, ). The blots shown are representative of 3 independent experiments.

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