RNA-Seq Expression Analysis of Chronic Asthmatic Mice with Bu-Shen-Yi-Qi Formula Treatment and Prediction of Regulated Gene Targets of Anti-Airway Remodeling

Figure 1

(a) The clustering heat maps of gene expressions. Red represents the upregulation expressions; blue represents the downregulation expressions. The color brightness is associated with differences in multiples. (b) The Venn diagram shows RNA-seq data for all genes that were regulated in asthma compared to control (M vs C) and by BSYQF treated compared to non-treated asthma controls (T vs M). FC: fold changes. Blue: M vs C, positive FC (FC > 1). Yellow: M vs C, negative FC (FC < 1). Green: M vs T, positive FC (FC > 1). Pink: M vs T, negative FC (FC < 1). (c). Volcano plot (M vs C). (d) Volcano plot (M vs T). Genes of interest are labeled as examples to show changes in expression levels. Horizontal dashed line represents expression value . Genes outside the midline have FC > 1.

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