RNA-Seq Expression Analysis of Chronic Asthmatic Mice with Bu-Shen-Yi-Qi Formula Treatment and Prediction of Regulated Gene Targets of Anti-Airway Remodeling

Table 1

Differently expressed genes showing the highest fold changes (in either direction) in lung samples of the mice in the control (C), asthmatic model (M), and BSYQF treatment (T) groups.

A. M vs C		B. T vs M		C
Gene	Fold change	Gene	Fold change	Gene	M vs C	T vs M
Increased		Increased		Timp1	6.22	−3.01
Chil4	11.74	A530016L24Rik	7.79	Reg1	6.20	−3.30
Sprr2a3	11.18	Tmem179	7.17	Dmp1	5.65	−4.15
Clca1	9.86	Serpinb11	7.01	Glod5	5.55	−5.52
Mcpt1	8.71	Ebf2	6.85	Rnf17	5.35	−5.33
Rnase2a	8.43	Htr3a	6.59	Dspp	5.14	−5.11
Capn9	7.48	Lep	6.52	Lep	−8.64	6.52
Saa3	7.42	Glycam1	6.43	A530016L24Rik	−8.36	7.79
Saa1	7.23	Syt4	6.39	Tmem179	−7.57	7.17
Saa4	7.18	Lrtm1	5.95	Lctl	−7.36	5.20
Retnlb	7.09	Nalcn	5.91	Ebf2	−6.77	6.85
A	6.93	Slc5a8	5.89	Nalcn	−6.42	5.91
Cd209e	6.93	Dsc3	5.84	Adipoq	−5.97	5.80
Mmp12	6.84	Adipoq	5.80	Htr3a	−5.58	6.59
Timd2	6.78	Lsmem1	5.76	Myh1	−5.57	4.94
Retnla	6.64	Ugt2b34	5.57	Car3	−5.50	5.10
Cbln1	6.47	Cartpt	5.54	Gm10382	−5.40	4.72
Mrgprg	6.32	Dnmt3c	5.51	Lrtm1	−5.39	5.95
Prss32	6.27	Nefm	5.46	Slc5a8	−5.29	5.89
Timp1	6.22	Slc38a3	5.42	Hist1h4k	−5.27	5.29
Reg1	6.20	Pnoc	5.39	Kcnq3	−5.26	5.22
Fgl1	6.18	Hist1h4k	5.29	Pnoc	−5.18	5.39
Btbd16	6.12	Krt6a	5.26	Dnmt3c	−5.16	5.51
Nuggc	6.11	Serpina1e	5.24	Retn	−5.09	4.96
Decreased		Acp7	5.24	Cfd	−5.06	5.01
Lep	−8.64	Krt5	5.23	Lsmem1	−5.03	5.76
A530016L24Rik	−8.36	Kcnq3	5.22	—	—
Tmem179	−7.57	BC117090	5.21
Lctl	−7.36	Lctl	5.20
Ebf2	−6.77	BC016579	5.19
Gys2	−6.69	Car3	5.10
Ebf3	−6.58	Gm5416	5.09
Nalcn	−6.42	Dync1i1	5.03
—	—	Cfd	5.01
		Decreased
		Glod5	−5.52
		Rnf17	−5.33
		Dspp	−5.11
		Gm49320	−5.03
		Azgp1	−5.03

A, all genes with increased or decreased expression levels by FC > (±)6 () in the asthma group (M) relative to the control group (C); B, all genes upregulated or downregulated by the BSYQF treatment group (T) with FC > =(±)5 () relative to the asthma group (M). C, all genes with FC > (±)5 () that were reverted in their expression towards normal levels by BSYQF treatment. Genes are ranked orderly by FC (positive or negative values).