RNA-Seq Expression Analysis of Chronic Asthmatic Mice with Bu-Shen-Yi-Qi Formula Treatment and Prediction of Regulated Gene Targets of Anti-Airway Remodeling
Table 1
Differently expressed genes showing the highest fold changes (in either direction) in lung samples of the mice in the control (C), asthmatic model (M), and BSYQF treatment (T) groups.
A. M vs C
B. T vs M
C
Gene
Fold change
Gene
Fold change
Gene
M vs C
T vs M
Increased
Increased
Timp1
6.22
−3.01
Chil4
11.74
A530016L24Rik
7.79
Reg1
6.20
−3.30
Sprr2a3
11.18
Tmem179
7.17
Dmp1
5.65
−4.15
Clca1
9.86
Serpinb11
7.01
Glod5
5.55
−5.52
Mcpt1
8.71
Ebf2
6.85
Rnf17
5.35
−5.33
Rnase2a
8.43
Htr3a
6.59
Dspp
5.14
−5.11
Capn9
7.48
Lep
6.52
Lep
−8.64
6.52
Saa3
7.42
Glycam1
6.43
A530016L24Rik
−8.36
7.79
Saa1
7.23
Syt4
6.39
Tmem179
−7.57
7.17
Saa4
7.18
Lrtm1
5.95
Lctl
−7.36
5.20
Retnlb
7.09
Nalcn
5.91
Ebf2
−6.77
6.85
A
6.93
Slc5a8
5.89
Nalcn
−6.42
5.91
Cd209e
6.93
Dsc3
5.84
Adipoq
−5.97
5.80
Mmp12
6.84
Adipoq
5.80
Htr3a
−5.58
6.59
Timd2
6.78
Lsmem1
5.76
Myh1
−5.57
4.94
Retnla
6.64
Ugt2b34
5.57
Car3
−5.50
5.10
Cbln1
6.47
Cartpt
5.54
Gm10382
−5.40
4.72
Mrgprg
6.32
Dnmt3c
5.51
Lrtm1
−5.39
5.95
Prss32
6.27
Nefm
5.46
Slc5a8
−5.29
5.89
Timp1
6.22
Slc38a3
5.42
Hist1h4k
−5.27
5.29
Reg1
6.20
Pnoc
5.39
Kcnq3
−5.26
5.22
Fgl1
6.18
Hist1h4k
5.29
Pnoc
−5.18
5.39
Btbd16
6.12
Krt6a
5.26
Dnmt3c
−5.16
5.51
Nuggc
6.11
Serpina1e
5.24
Retn
−5.09
4.96
Decreased
Acp7
5.24
Cfd
−5.06
5.01
Lep
−8.64
Krt5
5.23
Lsmem1
−5.03
5.76
A530016L24Rik
−8.36
Kcnq3
5.22
—
—
Tmem179
−7.57
BC117090
5.21
Lctl
−7.36
Lctl
5.20
Ebf2
−6.77
BC016579
5.19
Gys2
−6.69
Car3
5.10
Ebf3
−6.58
Gm5416
5.09
Nalcn
−6.42
Dync1i1
5.03
—
—
Cfd
5.01
Decreased
Glod5
−5.52
Rnf17
−5.33
Dspp
−5.11
Gm49320
−5.03
Azgp1
−5.03
A, all genes with increased or decreased expression levels by FC > (±)6 () in the asthma group (M) relative to the control group (C); B, all genes upregulated or downregulated by the BSYQF treatment group (T) with FC > =(±)5 () relative to the asthma group (M). C, all genes with FC > (±)5 () that were reverted in their expression towards normal levels by BSYQF treatment. Genes are ranked orderly by FC (positive or negative values).