The Aqueous Extract of Eucommia Leaves Promotes Proliferation, Differentiation, and Mineralization of Osteoblast-Like MC3T3-E1 Cells

Figure 2

Effects of ELAE on MC3T3-E1 cell proliferation. Cell viability was detected by CCK-8 assay after the treatment with ELAE at progressively increasing concentrations of 0, 0.05, 0.1, 0.15, 0.2, 0.25, and 0.3 mg/mL. Cell viability of the ELAE-treated groups (0.05, 0.1, 0.15, 0.2, 0.25, and 0.3 mg/mL) was estimated by normalizing to cell viability of the untreated group (0 mg/mL). Data are presented as the mean with standard deviation for technical triplicates in an experiment representative of several independent ones. and represent the differences of cell viabilities under ELAE treatment (Student’s t-test).