Saireito Improves Lymphatic Function and Prevents UVB-Induced Acute Inflammation and Photodamage in HR-1 Hairless Mice

Figure 6

Effect of SRT on UVB-induced structure change in lymphatic vessels and VEGF-C expression level. (a) Skin sections were collected at 24 h after irradiation, and immunohistochemical staining was performed for the lymphatic vessel marker podoplanin. Scale bar = 200 μm. (b) UVB irradiation increased lymphatic vessel size at 48 h after irradiation, and SRT had no effect on the dilation. (c) SRT suppressed UVB-induced decrease in lymphatic vessel density at 24 h after irradiation. (d) VEGF-C expression at 24 h after UVB irradiation was measured using western blot analysis. SRT upregulated the VEGF-C expression compared with the vehicle group at 24 h after irradiation. Data were expressed as mean ± SEM (n = 5). and vs. control, Dunnett’s test. vs. vehicle, Student’s t-test. SRT: Saireito extract; UVB: ultraviolet B; VEGF-C: vascular endothelial growth factor C.

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