Zingiberensis Newsaponin Inhibits the Malignant Progression of Hepatocellular Carcinoma via Suppressing Autophagy Moderated by the AKR1C1-Mediated JAK2/STAT3 Pathway

Figure 2

ZnS inhibits the viability and migration, as well as promotes apoptosis of HCC cells. (a) The viability of Huh7 and SMMC7721 cells assessed by CCK-8 assay. (b) The migration ability of Huh7 and SMMC7721 cells examined by transwell assay (400x magnification). (c) The apoptosis levels of Huh7 and SMMC7721 cells measured using flow cytometry assay. Cells were treated with 0.5 µM ZnS for Huh7 and 1.0 µM for SMMC7721 cells and/or 10 mM 3-methyladenine (3-MA).  < 0.01 and  < 0.001 vs. the control group.  < 0.01 and  < 0.001 vs. the ZnS + 3-MA group.

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