Anti-Inflammatory Effects of Heat-Processed Artemisia capillaris Thunberg by Regulating IκBα/NF-κB Complex and 15-PGDH in Mouse Macrophage Cells

Figure 5

Effects of HPAC on HO-1 via MAPK signaling. RAW 264.7 cells were treated with HPAC 200 μg/mL for indicated times. (a) Western blot analysis of HO-1 and β-actin. (b) Quantitative analysis of HO-1 expression relative to β-actin. RAW 264.7 cells were treated with indicated concentration of HPAC for 4 h. (c) Western blot analysis of HO-1, p-p38, p38, p-ERK, ERK, p-JNK, JNK, and β-actin. Quantitative analysis of (d) HO-1 expression relative to β-actin, (e) p-p38 expression relative to p38, (f) p-ERK expression relative to ERK, and (g) p-JNK expression relative to JNK. RAW 264.7 cells were pretreated with specific MAPK inhibitors, SB203580 (20 μM), PD98059 (30 μM), and SP600125 (20 μM), for 1 h and then HPAC 200 μg/mL for 4 h. (h) Western blot analysis of HO-1 and β-actin. (i) Quantitative analysis of HO-1 expression relative to β-actin. The data are presented as means ± SEM (n = 3). versus control group, , , and versus HPAC group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)