Anti-Inflammatory Effects of Heat-Processed Artemisia capillaris Thunberg by Regulating IκBα/NF-κB Complex and 15-PGDH in Mouse Macrophage Cells

Figure 6

Effects of HPAC on IkBα/NF-kB pathway by HO-1 activation. RAW264.7 cells were pretreated with MAPK specific inhibitors, SB203580 (20 μM), PD98059 (30 μM), and SP600125 (20 μM), for 1 h and then HPAC 200 ug/mL for 2 h followed by LPS for 2 h. (a) Western blot analysis of HO-1, p-IκBα, IκBα, and β-actin. Quantitative analysis of (b) p-IκBα expression relative to IκBα; (c) HO-1 expression relative to β-actin. (d) Nuclear and cytosolic extracts were isolated and levels of NF-κB p65 in nuclear fraction were determined using western blot analysis. (e) Quantitative analysis of NF-κB p65 expression relative to Lamin A\C. The data are presented as means ± SEM (n = 3). versus control group, , , and versus HPAC group.

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