A Comparative Study on Anticancer Effects of the Alhagi maurorum and Amygdalus haussknechtii Extracts Alone and in Combination with Docetaxel on 4T1 Breast Cancer Cells

Figure 2

Combination effect of DTX and A.m or A.h extract on 4T1 cells. ((a) and (b)) Isobologram analysis represents the synergistic effect of most of points for A.m + DTX combination. A.m concentrations > 100 µg/ml in combination mode represent antagonism. The combination index (CI) was calculated by CompuSyn software. Points above and below the isoeffect line reflect antagonism and synergy, respectively. 15 data points were entered for each combination. ((c) and (d)) The fraction affected (FA) versus CI curve: FA is defined as the growth inhibition affected by the dose. CI < 1, CI = 1, and CI > 1 indicate synergism, additive effect (solid line), and antagonism effects, respectively. (e) Effect of A.m extract on the IC50 value of DTX : 4T1 cells were treated with A.m + DTX at various concentrations for 48 h and then cell viability was assessed by MTT assay. Combined treatments resulted in significant decrease in IC50 of DTX at 15, 31, 51, 62, and 125 µg/ml of A.m. (f) Effect of A.h extract on the IC50 value of DTX: 4T1 cells were treated with A.h + DTX at multiple concentrations for 48 (h) and then cell viability was assessed by MTT assay. Combined treatments resulted in a significant decrease in IC50 of DTX at 62, 84, and, 125 µg/ml of A.h. Values are given as mean ± SD of three independent experiments. represent significant changes in the IC50 of DTX.

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