A Comparative Study on Anticancer Effects of the Alhagi maurorum and Amygdalus haussknechtii Extracts Alone and in Combination with Docetaxel on 4T1 Breast Cancer Cells

Figure 4

Flow cytometric analysis of apoptotic detection. (a, b) Effect of A.m and DTX alone and in combination with each other on induction of apoptosis of 4T1 cells. A.m + DTX (IC50 + IC25; IC50 + IC10; IC10 + IC10) combination groups had a bigger apoptotic effect compared to A.m extract alone. (c, d) Effect of individual and combinational A.h and DTX on induction of apoptosis of 4T1 cells. All combination groups had a bigger apoptotic effect compared to A.h extract alone, and A.h + DTX (IC50 + IC10) group had a bigger apoptotic effect compared to both of A.h and DTX alone. IC values were as follows: for A.m : IC10 = 32 µg/ml, IC25 = 40 µg/ml, and IC50 = 57 µg/ml; for A.h : IC10 = 10 µg/ml, IC25 = 33 µg/ml, and IC50 = 85 µg/ml; and for DTX : IC10 = 0.4 µM, IC25 = 3.5 µM, and IC50 = 11 µM.

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