Shen Shuai II Recipe Attenuates Renal Interstitial Fibrosis by Improving Hypoxia via the IL-1β/c-Myc Pathway

Figure 4

Shen Shuai II Recipe (SSR) inhibits interleukin- (IL-) 1β expression in NRK-52E cells under hypoxia. (a) Effect of SSR on the viability of NRK-52E cells (n = 5). (b) Concentration of IL-1β (n = 4). (c) Representative photomicrographs of IL-1β expression detected by immunocytochemistry. Original magnification, ×200. (d) Representative western blotting bands of hypoxia-inducible factor-1α (HIF-1α), IL-1β, fibronectin (FN), and collagen- (COL-) I proteins in the NRK-52E cells and target protein/β-ACTIN ratios, including HIF-1α/β-actin, IL-1β/β-actin, FN/β-actin, and COL-I/β-actin ratios (n = 4). Values are mean ± SE.

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