Fraxetin Inhibits the Proliferation and Metastasis of Glioma Cells by Inactivating JAK2/STAT3 Signaling

Figure 4

Fraxetin inhibits JAK2/STAT3 signaling in U251 cells. ((a), (b), and (c)) Western blot analysis of key proteins involved in JAK2/STAT3 signaling. Cells were treated with fraxetin (0 μM, 100 μM, and 200 μM) for 24 h. (d) Immunofluorescence analysis of STAT3. Cells were treated with fraxetin (0 μM, 100 μM, and 200 μM) for 24 h. ((e) and (f)) qRT-PCR analysis of IL-6 and IL-10. Cells were treated with fraxetin (0 μM, 100 μM, and 200 μM) for 24 h. (g) CCK8 assay. Cells were treated with fraxetin (200 μM) and Colivelin (10 μM) together or fraxetin (200 μM) alone for 24 h. (h) Transwell invasive assay. Cells were treated with fraxetin (200 μM) and Colivelin (10 μM) together or fraxetin (200 μM) alone for 24 h. ; ; .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)