Inhibitory Effects of Euphorbia ebracteolata Hayata Extract ECB on Melanoma-Induced Hyperplasia of Blood Vessels in Zebrafish Embryos

Figure 2

Euphorbiaceae compound B (ECB) inhibited SIV ectopic hyperplasia induced by melanoma cells in transgenic Tg (flk1:GFP) zebrafish embryos. Human melanoma cells were microinjected into zebrafish embryos at 48 hpf, and the resulting xenografts were imaged with a fluorescence microscope after 24 and 48 h (hpi), respectively. (a, e) SIV ectopic vessels in green fluorescence (red arrows) at 24 hpi zebrafish embryo (control). (b, f) SIV ectopic vessels (red arrows) at 48 hpi zebrafish embryo (control). (c, g) SIV ectopic vessels after treatment with 20 µg/mL ECB for 48 h. (d, h) SIV ectopic vessels after treatment with 1 µM PTK for 48 h. (i) The length of ectopic blood vessels in A2058 or HUVEC xenografts at 24 and 48 hpi and the effects after treatment with ECB and PTK (). (j) The number of ectopic blood vessels in A2058 or HUVEC xenografts at 24 hpi and 48 hpi and the effects after treatment with ECB or PTK (). Scale bar = 200 µm.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)