Duchesnea indica Extract Attenuates Coal Fly Ash-Induced Inflammation in Murine Alveolar Macrophages through the NF-Kappa B Pathway

Figure 6

Effect of the DIE on CFA-induced NF-κB translocation in MH-S cells. The cells were seeded on coated cover slip in a six-well plate, and the groups were as follows: basal group, only CFA (2.5 μg/mL)-induced group, CFA with the DIE (100 μg/ml) group, and CFA with Bay-11 (inhibitor of p-NF-κB) group. The cells were treated with the DIE and Bay-11 (10 µM) for 30 min prior to the CFA treatment (2.5 μg/mL) and were incubated for 18 h. The nuclear translocation of p-NF-κB was analyzed via immunofluorescence staining. The samples were mounted using ProLong™ Gold Antifade Reagent with DAPI to visualize the nuclei (blue). Stained cells were analyzed via confocal microscopy (ZEISS) at 1000x magnification.