Induction of Apoptosis in HeLa Cells by a Novel Peptide from Fruiting Bodies of Morchella importuna via the Mitochondrial Apoptotic Pathway

Figure 6

Mitochondrial membrane potential assay. The cells were seeded in a 96-well microtiter plate cultivated under standard conditions for 12 h then different concentrations (50, 100, and 200 μg/mL) of MIPP were added, and cells were cultured for another 12 h JC-1 (final concentration: 2.5 μg/mL per well) was added to the cells for 15 min. Intensity of fluorescence was recorded using a FACS Calibur flow cytometer. (a–d) The fluorescence immune stain of Hela cells through JC-1 method. (a) Negative control. (b–d) Different concentrations of MIPP, 50, 100, and 200 μg/mL, respectively. (e) The semiquantitatively analysis for the fluorescence immune stain. Scale bar = 100 μm. and , treated versus control group by one-way ANOVA Dunnett’s multiple comparison test.

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