Ultrafiltration Extract of Radix Angelica Sinensis and Radix Hedysari Attenuates Risk of Low-Dose X-Ray Radiation-Induced Myocardial Fibrosis In Vitro

Figure 1

Identification of CFs and RAS-RH inhibited the proliferation of cardiac fibroblasts after X-ray radiation. (a, b) Representative images of identification of CFs by immunofluorescence staining of α-SMA actin and Vimentin were used to identify the purity of myocardial fibroblasts isolated by double enzyme digestion. Nuclei were stained blue; α-SMA and Vimentin were stained red, magnification ×200. (c) Representative results of shape of CFs by fluorescence microscope in the control group and X-ray group (at 48h after irradiation). Magnification ×40. (d) RAS-RH inhibits proliferation rate of primary CFs after X-ray radiation depended on the manner of dose-time. CFs were treated with 3, 6, and 9 μg/ml of RAS-RH after X-ray irradiation for the indicated times. Proliferation rate of CFs was determined by CCK-8 assay (n = 5, , vs. the control group, , vs. X-ray group). (e) TGF-β1-positive cells in each group with immunofluorescence staining. Nuclei were stained blue and TGF-β1 stained red, magnification ×200.

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