Evodiae fructus Extract Inhibits Interleukin-1β-Induced MMP-1, MMP-3, and Inflammatory Cytokine Expression by Suppressing the Activation of MAPK and STAT-3 in Human Gingival Fibroblasts In Vitro

Figure 4

EFE inhibits MAPK activation by IL-1β in HGFs. Cells were preincubated with the EFE for 1 h and then stimulated with IL-1β for 30 min. Using western blotting, the phosphorylated and total MAPK (p38, ERK, and JNK) proteins were quantified (a). Cells were prestimulated with 25 and 50 μg/ml of the EFE for 1 h and then incubated with 0.5 ng/ml of IL-1β for 3 h. After nuclear fractionation, western blot analysis was performed to quantify nuclear NF-κB (p65 and p50) and AP-1 (p-c-Jun) subunits (b).

(a)

(b)