Paeoniflorin Improved Constipation in the Loperamide-Induced Rat Model via TGR5/TRPA1 Signaling-Mediated 5-Hydroxytryptamine Secretion

<div>5-HT release and Tph-1 expression were hindered by TGR5/TRPA1 signaling deactivation. (a) The CCK-8 assay was used to test the cell proliferation of RIN-14B cells at 24 hours. (b) The 5-HT secretion in the cell supernatant of RIN-14B cells was assayed by ELISA. The expression of Tph-1 in RIN-14B cells was determined via western blot (c) and IF stain (d, e). Scale 50 <i>μ</i>m. 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Evidence-Based Complementary and Alternative Medicine

fig7

Figure 7

Figure 7: Paeoniflorin Improved Constipation in the Loperamide-Induced Rat Model via TGR5/TRPA1 Signaling-Mediated 5-Hydroxytryptamine Secretion 