Effects of Sinapic Acid Combined with Cisplatin on the Apoptosis and Autophagy of the Hepatoma Cells HepG2 and SMMC-7721

Figure 10

Inhibition of autophagy enhances the Sa/DDP-induced apoptosis of the HepG2 and SMMC-7721 cells. The hepatic cancer cells were pretreated with the apoptosis inhibitor 20 µM Z-VAD-FMK or the autophagy inhibitor 40 µM HCQ for 1 h. The HepG2 and SMMC-7721 cells coincubated with Sa (0 and 600 µM) and 5 µM DDP for 72 h were observed for morphological changes and Hoechst (blue) staining MDC (green) staining by using a fluorescence microscope (40x magnification).