Effects of Sinapic Acid Combined with Cisplatin on the Apoptosis and Autophagy of the Hepatoma Cells HepG2 and SMMC-7721

Figure 11

Inhibition of autophagy enhances the Sa/DDP-induced apoptosis of the HepG2 and SMMC-7721 cells. The hepatic cancer cells were pretreated with the apoptosis inhibitor 20 µM Z-VAD-FMK or autophagy inhibitor 40 µM HCQ for 1 h. Western blot was used to analyze the expression in the HepG2 and SMMC-7721 cells coincubated with Sa (0 and 600 µM) and 5 µM DDP for 72 h of apoptosis-related proteins caspase-3 and autophagy-related proteins LC3II, with β-actin as the internal reference. All results were exhibited as the means ± SD of three different samples. ^a–gBars with different superscript letters differ significantly ( < 0.05) by Duncan’s multiple range test.