Evidence-Based Complementary and Alternative Medicine

Research Article

GC/MS Profiling and Ex Vivo Antibacterial Activity of Salvadora persica (Siwak) against Enterococcus faecalis as Intracanal Medicament

Table 1

The chemical profile of SPE.


Peak #	R_t	Component	Molecular formula	RI_exp^a	RI_lit^b	Content (%)	Identification^c

1.	18.35	Carvacrol	C₁₀H₁₄O	1288	1288	0.57	MS, KI
2.	20.47	Benzyl isothiocyanate	C₈H₇NS	1361	1359	33.32	MS, KI
3.	21.14	n-Tetradecane	C₁₄H₃₀	1384	1400	0.21	MS, KI
4.	23.76	n-Pentadecane	C₁₅H₃₂	1485	1500	0.38	MS, KI
5.	26.24	n-Hexadecane	C₁₆H₃₄	1582	1600	0.13	MS, KI
6.	34.40	n-Hexadecanoic acid (palmitic acid)	C₁₆H₃₂O₂	1948	1946	4.27	MS, KI
7.	34.91	Hexadecanoic acid ethyl ester	C₁₈H₃₆O₂	1972	1975	0.13	MS, KI
8.	36.12	Cyclic octaatomic sulphur	S₈	2034	2055	0.99	MS, KI
9.	37.86	Oleic acid	C₁₈H₃₄O₂	2130	2130	1.95	MS, KI
10.	37.92	(Z)-11-Octadecenoic acid	C₁₈H₃₄O₂	2133	2117	3.16	MS, KI
11.	38.15	(Z,Z)-9,12-Octadecadienoyl chloride (linoleic acid chloride)	C₁₈H₃₁ClO	2146	2139	0.37	MS, KI
12.	38.24	(Z)-9-Octadecenoic acid ethyl ester (oleic acid ethyl ester)	C₂₀H₃₈O₂	2151	2168	0.45	MS, KI
13.	38.36	9-Octadecenoic acid ethyl ester	C₂₀H₃₈O₂	2157	2171	0.14	MS, KI
14.	42.84	p-Cresol, 2,2′-methylene bis [6-tert-butyl-	C₂₃H₃₂O₂	2403	2398	0.13	MS, KI
15.	48.95	Squalene	C₃₀H₅₀	2796	2814	0.17	MS, KI
16.	49.51	N-Benzylpalmitamide	C₂₃H₃₉NO	2832	2880	0.36	MS
17.	51.09	Stigmasta-3,5-diene	C₂₉H₄₈	2934	—	0.77	MS
18.	51.69	Stigmasterol acetate	C₃₁H₅₀O₂	2972	2879	0.12	MS, KI
19.	51.87	3β-Acetoxystigmasta-4,6,22-triene	C₃₁H₄₈O₂	2984	-	0.24	MS
20.	52.06	n-Benzyloctadecenamide (n-benzyloleamide)	C₂₅H₄₁NO	2996	2991	0.53	MS, KI
21.	52.17	Stigmasta-5,22-dien-3-ol, acetate	C₃₁H₅₀O₂	3003	-	0.65	MS
22.	52.47	Clionasterol acetate	C₃₁H₅₂O₂	3022	-	0.50	MS
23.	52.66	Stigmastan-3,5,22-triene	C₂₉H₄₆	3034	2981	1.89	MS, KI
24.	52.94	β-Sitosterol acetate	C₃₁H₅₂O₂	3052	-	2.28	MS
25.	53.28	Cholesterol	C₂₇H₄₆O	3074	3075	0.66	MS, KI
26.	54.89	Campesterol	C₂₈H₄₈O	3177	3131	0.70	MS
27.	55.40	Stigmasterol	C₂₉H₄₈O	3210	3213	5.92	MS, KI
28.	56.44	γ-Sitosterol	C₂₉H₅₀O	3277	3290	25.76	MS, KI
29.	57.47	Unidentified	-	3344	-	3.57	-
30.	58.21	3,5-Stigmastadien-7-one	C₂₉H₄₆O	3391	-	0.56	MS
31.	59.04	Sitostenone	C₂₉H₄₈O	3444	3435	0.54	MS, KI
32.	60.19	Lupeol	C₃₀H₅₀O	3518	3451	1.24	MS
	% total identified			89.09

Compounds are listed in order of their elution times on Rtx-5MS column. ^aRI_exp, retention index determined experimentally relative to C8 – C28 n-alkanes on Rtx-5MS column; ^bRI_lit, published retention indices; ^cIdentification, was based on comparison of the compounds’ mass spectral data (MS) and retention indices (RI) with those of NIST Mass Spectral Library (2017), Wiley Registry of Mass Spectral Data 8^th edition and literature. Bold shows major components.