Ethanolic Fruit Extract of Emblica officinalis Suppresses Neuroinflammation in Microglia and Promotes Neurite Outgrowth in Neuro2a Cells

Figure 8

Effect of EO crude extract on the mRNA expression level of two neuronal markers in differentiated Neuro2a cells. Cells were treated with LPS (1 μg/mL) or EO crude fruit extract (0.5 or 1 μg/mL). After 24 h, the cells were collected, and mRNA expression levels of two neuronal markers ((a) TuJ1 gene and (b) MAP2 gene) were tested by RT-qPCR. (c) TuJ1 protein expression was measured by Western blot. The results are presented as mean ± SD. ( and , compared with the LPS-treated group.)

(a)

(b)

(c)