[Retracted] A Nanoparticle-Conjugated Anti-TBK1 siRNA Induces Autophagy-Related Apoptosis and Enhances cGAS-STING Pathway in GBM Cells

Figure 5

TBK1si/rGO-PEG exerted antitumor effects in vitro. (a) CCK8 analysis cell viability. When U251 cells were transfected with NS, rGO-PEG, Consi/rGO-PEG, or TBK1si/rGO-PEG for 3, 24, 48, and 72 hours, cell viability was tested by the CCK8 test. (b) Cell cycle analysis of U251 cells after 48-hour intervention with TBK1si/rGO-PEG. (c) Percentage of U251 cells in each mitotic phase after treatment with NS, rGO-PEG, Consi/rGO-PEG, or TBK1si/rGO-PEG. (d)-(e) When U251 cells were transfected with NS, rGO-PEG, Consi/rGO-PEG, or TBK1si/rGO-PEG for 72 hours, U251 cells were collected and stained with Annexin V-CF Blue/PI and then analyzed by flow cytometry. Statistics of GBM cell apoptosis. PI, propidium iodide. The result was a representative of three independent experiments. Error bars represent mean ± SD. P values were determined by one-way analysis of variance (ANOVA) followed by Tukey post hoc test.  < 0.05.  < 0.01.  < 0.001.

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