Yixintongmai Inhibits Proliferation and Migration and Promotes Apoptosis of Vascular Smooth Muscle Cells Cultured with High Glucose

Figure 1

Effects of yixintongmai on proliferation and PCNA expression of VSMCs cultured with high glucose. NG: normal glucose (5.5 mM); MG: normal glucose (5.5 mM) and mannitol (19.5 mM); HG: high glucose (25 mM); HG + Y: high glucose (25 mM) and yixintongmai powder (360 μg/ml); VSMCs proliferation were evaluated by CCK-8 (a) and cell counting (b); Western blot of PCNA expression (c) was quantified by densitometry using ImageJ V1.8.0 (d); densitometry quantification values are shown by fold over NG or HG. Mannitol was used as an osmolality control for high glucose condition. Values were expressed as means ± SD of sextuplicate experiments. ; ; NS: not significant ().

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