Efficacy of Ethanolic Extract of Syzygium aromaticum in the Treatment of Multidrug-Resistant Pseudomonas aeruginosa Clinical Isolates Associated with Urinary Tract Infections

Figure 2

Agarose gel electrophoresis of multiplex PCR of positive P. aeruginosa isolates (6 isolates) carrying antibiotic-resistant genes. (a) bla_TEM antibiotic-resistant gene; lanes 1–6: P. aeruginosa isolates P14, P13, P12, P9, P8, and P4 at 516 bp, lane 7: negative control for detected genes; lane 8: positive control of DNA confirmed by reference laboratory for quality control; lane 9: 100 bp ladder as molecular size DNA marker (cat. no. 239035) supplied from QIAGEN (USA). (b) bla_SHV antibiotic-resistant gene; lanes 6 and 7: only 2 P. aeruginosa P8 and P9 were bla_SHV positive; lane 4: positive control of DNA confirmed by reference laboratory for quality control; lane 5: 100 bp ladder as molecular size DNA marker (cat. no. 239035) supplied from QIAGEN (USA). Other lanes were negative bla_SHV results. (c) bla_CTX antibiotic-resistant gene; all P. aeruginosa isolates were bla_CTX negative; lane 1: 100 bp ladder as molecular size DNA marker (cat. no. 239035) supplied from QIAGEN (USA) lane 2: positive control of DNA.

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