Efficacy of Ethanolic Extract of Syzygium aromaticum in the Treatment of Multidrug-Resistant Pseudomonas aeruginosa Clinical Isolates Associated with Urinary Tract Infections

Figure 3

Agarose gel electrophoresis of multiplex PCR of virulence genes characterized for P. aeruginosa isolates (6 isolates). Virulence-associated genes were detected in all P. aeruginosa isolates at 396, 1220, 656, and 180 bp in toxA, lasB, pslA, and fliC, respectively. (a) toxA and (d) fliC virulence genes; lane 7: negative control for detected genes; lane 8: positive control of DNA confirmed by reference laboratory for quality control; lane 9: gel pilot 100 bp ladder as molecular size DNA marker (cat. no. 239045) supplied from QIAGEN (USA). (b) lasB virulence gene; lane 3: negative control for detected genes; lane 2: positive control of DNA confirmed by reference laboratory for quality control; lane 1: gel pilot 100 bp ladder as molecular size DNA marker (cat. no. 239045) supplied from QIAGEN (USA). (c) pslA virulence gene; lane 9: negative control for detected genes; lane 8: positive control of DNA confirmed by reference laboratory for quality control; lane 7: gel pilot 100 bp ladder as molecular size DNA marker (cat. no. 239045) supplied from QIAGEN (USA).

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