Gualou Guizhi Granule Protects against OGD/R-Induced Injury by Inhibiting Cell Pyroptosis via the PI3K/Akt Signaling Pathway

Table 1

Primers used for quantitative real-time PCR analysis.

Gene	Forward primer	Reverse primer
IL-1β	5′-ATG ACC TGT TCT TTG AGG CTG AC-3′	5′-CGA GAT GCT GCT GTG AGA TTT G-3′
IL-18	5′-CAT GCC ATG GCT GCT GAA CCA GTA GAA GA-3′	5′-CGG GAT CCA ATA GCT AGT CTT CGT TTT G-3′
NLRP3	5′-CCAGACCTCCAAGACCACGACT-3′	5′-ATCCGCAGCCAATGAACAGA-3′
Caspase-1	5′-TGG TCT TGT GAC TTG GAG GA-3′	5′-TGG CTT CTT ATTGGC ACG AT-3′
ASC	5′-AGT TTC ACA CCA GCC TGGAA-3′	5′-TTT TCA AGC TGG CTT TTC GT-3′
PI3K	5′-CACCACCCAAGCCCACTTCTAT-3′	5′-TTCCTCGCAATAGGTTCTCGGC-3′
Akt	5′-GTGGCAAGATGTGTATGAGAAGAAG-3′	5′-GCTGAGTAGGAGAACTGGGGAAAG-3′
GAPDH	5′- AGC CCA GAA CAT CAT CCC TG-3′	5′- AGC CCA GAA CAT CAT CCC TG-3′