Paeoniflorin Attenuates Dexamethasone-Induced Apoptosis of Osteoblast Cells and Promotes Bone Formation via Regulating AKT/mTOR/Autophagy Signaling Pathway

Figure 1

Effect of paeoniflorin on the viability and osteogenic differentiation of MC3T3-E1 induced by DEX (a, b). The cells were incubated with different concentrations of dexamethasone (DEX) and paeoniflorin (Pa) for 24 hours alone. Cell viability was determined by the Cell Counting Kit-8(CCK-8) assay. Measurements were in triplicate and data are presented as the mean ± SD.  < 0.05;  < 0.01 vs. control. (c) MC3T3-E1 cells were pretreated with different concentrations of paeoniflorin for 4 hours and then were incubated with 200 μM DEX for 24 hours. Cell viability was measured by the CCK-8 assay.  < 0.01 vs. control;  < 0.01 vs. DEX 200 μM. Effect of paeoniflorin and 200 μM DEX on alkaline phosphate (ALP) activity (d), alkaline phosphate staining (e), and Alizarin Red staining (f) in MC3T3-E1 cells. Data are presented as the mean ± SD.  < 0.01 vs. control;  < 0.01 vs. DEX 200 μM.

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