Paeoniflorin Attenuates Dexamethasone-Induced Apoptosis of Osteoblast Cells and Promotes Bone Formation via Regulating AKT/mTOR/Autophagy Signaling Pathway

Figure 3

Regulations of AKT/mTOR pathway and autophagy by paeoniflorin after interventions of DEX in MC3T3-E1 cells. (a, b) Expressions of proteins involved in the AKT/mTOR pathway and autophagy were detected by western blot analysis. Data are presented as the mean ± SD.  < 0.05;  < 0.01 vs. control;  < 0.05 vs. DEX;  < 0.01 vs. DEX. (c) Expressions of representative autophagic mRNA as SQSTM1, ULK1, and Atg5 were determined by the measurements of RT-qRCR.  < 0.05,  < 0.01 vs. control;  < 0.05 vs. DEX. (d, e) AKT agonist-SC79 was pretreated with MC3T3-E1 cells to identify the inhibition effect of AKT/mTOR pathway by paeoniflorin. Expression of related proteins was measured by western blot. Quantitated levels of protein were statistically evaluated.  < 0.05;  < 0.01 vs. control;  < 0.05;  < 0.01 vs. DEX.

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