Total Flavonoids of Rhizoma Drynariae Restore the MMP/TIMP Balance in Models of Osteoarthritis by Inhibiting the Activation of the NF- and PI3K/AKT Pathways

Figure 7

Inhibition of MMP-3 and MMP-13 and promotion of TIMP-4 protein expression in IL-1β-induced SW1353 cells by total flavonoids of Rhizoma Drynariae (TFRD). Before stimulation with 10 ng/mL IL-1β for 12 h, cells were pretreated with 150, 300, or 600 μg/mL TFRD or 100 μmol/L dexamethasone (DEX) for 1 h. The cells were lysed, and MMP-1, MMP-3, MMP-13, and TIMP-4 levels were measured by Western blotting. The data are derived from three independent experiments and expressed as the mean ± standard deviation. ( < 0.05 compared with the control group; ^# < 0.05 compared with the IL-1β-treated group).