Total Flavonoids of Rhizoma Drynariae Restore the MMP/TIMP Balance in Models of Osteoarthritis by Inhibiting the Activation of the NF- and PI3K/AKT Pathways

Table 1

Prime sequences for quantitative real-time PCR.

Gene name	Sequence (5′-to-3′)
Human MMP-1 sense	CTGTTTTCTGGCCACAACTG
Human MMP-1 antisense	GGAAGCCAAAGGAGCTGTAG
Human MMP-3 sense	TTGGCCATCTCTTCCTTCAG
Human MMP-3 antisense	GAAACCTAGGGTGTGGATGC
Human MMP-13 sense	GTGCCCTTCTTCACACAGAC
Human MMP-13 antisense	AGAGCAGACTTTGAGTCATTGC
Human TIMP-4 sense	CACTCGGCACTTGTGATTCG
Human TIMP-4 antisense	GTTTGATTTCATACCGGAGCA
Human NF- p65 sense	AGGAGCACAGATACCACCAAGACC
Human NF- p65 antisense	AAGCAGAGCCGCACAGCATTC
Human AKT sense	CACGTCGGAGACTGACACC
Human AKT antisense	GCTGGCCGAGTAGGAGAAC
Human β-actin sense	TGGTGAAGACGCCAGTGGA
Human β-actin antisense	GCACCGTCAAGGCTGAGAAC
Rat MMP-1 sense	GCTTAGCCTTCCTTTGCTGTTGC
Rat MMP-1 antisense	GACGTCTTCACCCAAGTTGTAGTAG
Rat MMP-3 sense	CGGTGGCTTCAGTACCTTTC
Rat MMP-3 antisense	ACCTCCTCCCAGACCTTCA
Rat MMP-13 sense	TGCATACGAGCATCCATCCC
Rat MMP-13 antisense	CTCAAAGTGAACCGCAGCAC
Rat TIMP-4 sense	AGAAGGTAGTCCCTGCCAGT
Rat TIMP-4 antisense	CTTGGCCTTCTCGAACCCTT
Rat β-actin sense	CACCCGCGAGTACAACCTTC
Rat β-actin antisense	CCCATACCCACCATCACACC

MMP, matrix metalloproteinase; TIMP, tissue inhibitors of matrix metalloproteinase.