Involvement of Muscarinic Receptors in Hypotensive and Diuretic Effects of Aqueous Soluble Fraction from Asphodelus tenuifolius Cav.
Table 3
Effect of A. tenuifolius extract/fractions on urine and electrolyte excretion.
Treatment (mg/kg)
Urine volume (ml/100 gm)
Na+ (mEq/L)
K+ (mEq/L)
pH
2 h
4 h
6 h
NS
1.42 ± 0.30
2.39 ± 0.05
3.5 ± 0.12
113.6 ± 13
40 ± 1.2
6.89 ± 0.12
HCTZ 10 mg/kg
2.8 ± 0.43
4.04 ± 0.34
7.14 ± 0.6
173 ± 11
56 ± 5.1
6.92 ± 0.72
AT-Cr 100 mg/kg
1.3 ± 0.25
2.54 ± 0.39
3.35 ± 0.5
128.3 ± 3
45.3 ± 4.8
6.65 ± 0.27
AT-Cr 200 mg/kg
2.44 ± 0.20
3.25 ± 0.42
5.5 ± 0.59
135 ± 3
52.3 ± 3.9
6.71 ± 0.18
AT-Cr 300 mg/kg
2.55 ± 0.38
3.44 ± 0.7
6.39 ± 0.8
160 ± 2
59.3 ± 8.6
6.69 ± 0.45
AS-AT 100 mg/kg
2.60 ± 0.44
4.03 ± 0.31
5.28 ± 0.4
121 ± 4
45 ± 2.3
6.71 ± 0.98
AS-AT 200 mg/kg
2.73 ± 0.46
4.57 ± 0.34
5.68 ± 0.52
132 ± 3
52.3 ± 6.1
6.78 ± 0.29
AS-AT 300 mg/kg
2.86 ± 0.10
4.96 ± 0.75
7.19 ± 0.7
221 ± 18
66 ± 11
6.91 ± 0.23
BS-AT 100 mg/kg
1.74 ± 0.06
2.31 ± 0.60
3.77 ± 0.26
117.6 ± 2.2
39.6 ± 7.09
6.70 ± 0.23
BS-AT 200 mg/kg
1.90 ± 0.10
3.05 ± 0.20
4.89 ± 0.22
125 ± 1.5
43 ± 2.5
6.69 ± 0.18
BS-AT 300 mg/kg
2.28 ± 0.17
3.21 ± 0.23
5.67 ± 0.22
131 ± 5
47.6 ± 2.9
6.74 ± 0.89
DS-AT 100 mg/kg
1.33 ± 0.17
2.81 ± 0.30
4.02 ± 0.47
121 ± 8
40.3 ± 7.5
6.56 ± 0.45
DS-AT 200 mg/kg
2.04 ± 0.69
3.12 ± 0.36
5.1 ± 0.49
130 ± 15
43.3 ± 4.9
6.61 ± 0.78
DS-AT 300 mg/kg
2.39 ± 0.88
3.44 ± 0.45
5.69 ± 0.69
135 ± 3
42.3 ± 3.9
6.68 ± 0.40
Results are stated as mean ± SEM where ∗ = < 0.05, ∗∗ = < 0.01, and ∗∗∗ = < 0.001, as compared to normal saline- (NS-) treated group. HCTZ = hydrochlorothiazide, AT-Cr = crude extract of A. tenuifolius, AS-AT = aqueous soluble fraction of A. tenuifolius, BS-AT = butanol soluble fraction of A. tenuifolius, and DS-AT = dichloromethane soluble fraction of A. tenuifolius.
