Involvement of Muscarinic Receptors in Hypotensive and Diuretic Effects of Aqueous Soluble Fraction from Asphodelus tenuifolius Cav.

Table 4

Effect of A. tenuifolius extract/fractions on the diuretic index, Saluretic index, Lipschitz value, and excretion load.

Treatment (mg/kg)	Na/k	Diuretic index (DI)	Saluretic index Na^+ (SINa)	Saluretic index K^+ (SIK)	Saluretic index (SI)	Lipschitz value (LV)	EL Na^+	EL K^+
NS	2.84	1.00	1.00	1.00	1	0.49	1.003	0.310
HCTZ 10 mg/kg	4.80	2.03	1.53	1.4	1.465	1.00	3.287	1.11
AT-Cr 100 mg/kg	2.83	0.954	1.129	1.132	1.130	0.46	1.19	0.42
AT-Cr 200 mg/kg	2.58	1.581	1.188	1.307	1.247	0.77	2.02	0.78
AT-Cr 300 mg/kg	2.69	1.820	1.411	1.482	1.445	0.86	2.72	1.008
AS-AT 100 mg/kg	2.68	1.503	1.065	1.125	1.095	0.78	1.69	0.63
AS-AT 200 mg/kg	2.93	1.617	1.161	1.307	1.484	0.70	1.98	0.784
AS-AT 300 mg/kg	3.348	2.04	1.948	1.65	1.75	0.78	4.20	1.254
BS-AT 100 mg/kg	2.95	1.077	1.035	0.99	1.012	0.528	1.23	0.414
BS-AT 200 mg/kg	2.90	1.39	1.10	1.075	1.087	0.68	1.69	0.58
BS-AT 300 mg/kg	2.75	1.62	1.15	1.19	1.17	0.79	2.06	0.749
DS-AT 100 mg/kg	2.57	1.144	1.070	1.182	1.125	0.56	1.33	0.52
DS-AT 200 mg/kg	2.74	1.566	1.237	1	1.118	0.77	2.10	0.60
DS-AT 300 mg/kg	2.59	1.621	1.188	1.307	1.247	0.79	2.02	0.784

NS = normal saline, HCTZ = hydrochlorothiazide, AT-Cr = crude extract of A. tenuifolius, AS-AT = aqueous soluble fraction of A. tenuifolius, BS-AT = butanol soluble fraction of A. tenuifolius, DS-AT = dichloromethane soluble fraction of A. tenuifolius. DI = urine volume of extract-treated group/urine volume of NS-treated group. SI_Na = urine Na⁺ excretion of extract-treated group/urine Na⁺ excretion of NS-treated group, SI_K = Urine K⁺ excretion of extract-treated group/urine K⁺ excretion of NS-treated group, SI = SI_Na + SI_K of treated group/SI_Na + SI_K of the control group, LV = urine volume of extract-treated group/urine volume of HCTZ-treated group, Excretion load (EL) = electrolytes concentration (mEq/l) × urinary flow (ml/min).