Da Cheng Qi Decoction Alleviates Cerulein-Stimulated AR42J Pancreatic Acinar Cell Injury via the JAK2/STAT3 Signaling Pathway

Figure 4

DCQD suppressed JAK2/STAT3 signaling pathway in a concentration-dependent manner. (a) Real-time PCR was performed to detect the mRNA expression of JAK2 and STAT3. (b) Western blot was performed to detect the protein level of JAK2, p-JAK2, STAT3, and p-STAT3. (c) Immunofluorescence staining (400×) was performed to detect the location of p-STAT3 in cells. GAPDH was as the loading control for band density normalization. Data are presented as the means ± SD, n = 3.Immunofluorescence micrographs from one representative experiment out of three independent experiments are shown. All bar graphics:  < 0.05,  < 0.01 vs. the model group.

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